For CRISPR, tweaking DNA fragments before inserting yields highest efficiency rates yet

Researchers have now achieved the highest reported rates of inserting genes into human cells with the CRISPR-Cas9 gene-editing system, a necessary step for harnessing CRISPR for clinical gene-therapy applications. By chemically tweaking the ends of the DNA to be inserted, the new technique is up to five times more efficient than current approaches.

from Health & Medicine News -- ScienceDaily https://www.sciencedaily.com/releases/2019/12/191223122915.htm